Kursöversikt

 

Course content:

 

1. Molecular structure techniques

- x ray methods

- single molecule methods

- cryo electron microscopy/tomography

 

1. Chemical analysis across tissues

- matrix assisted laser desorption ionisation mass spectrometry

- secondary ion mass spectrometry imaging

- analysis of biopsies with liquid chromatography/mass spectrometry

- immunohistochemistry (fluorescence and mass spectrometry)

 

1. Cell and subcellular molecular analysis

- secondary ion mass spectrometry imaging (SIMS and NanoSIMS)

- electrochemical analysis of cells and organelles

- quantitative fluorescence analysis of cells and organelles

- nerve cell examples

 

1. Protein analysis

- quantitative proteomics

- molecular level with MRI

- molecular level with PET

 

5. Objectives

The theoretical and laboratory factors of the course are adjacent to current research and the intention is to prepare students for a degree project in neurobiochemistry or for further research studies in the subject. The materials in the lectures will be supported by several laboratory experiments, (video in the pandemic) and by observation, with short written reports. Each student will present a scientific paper to the class (20 min + 20 min discussion).

At the end of the course, Bioanalytical chemistry - single molecules to tissues, is expected to enable the student to:

• account for in-depth understanding of analytical methods used to measure molecules in life systems
• to become familiar with modern analysis in life sciences including imaging and prepare for further research studies in the subject
• critically consider the choices and limitations of different experimental methods for analysis in life science from tissues all the way to nanometer organelles
• acquire an in-depth understanding of the analysis of biomolecules in neuronal cells and tissues
• interpret and discuss laboratory results and draw reasonable conclusions

6. Course literature

A separate literature list will be provided.

7. Forms of assessment

Knowledge control for the course ‘bioanalytical chemistry - single molecules’ to tissues will takes place through a written examination and the written reports as well as the presentation. The laboratory observation days are compulsory as well as reports on the experimental procedures, results, and limitations.

Students who have been disapproved twice on the same examination are entitled to change the examiner, if practicable. Such a request shall be submitted to the institution and shall be in writing.

8. Rating

A written exam will be organized at the end of the course plus written laboratory reports. Students will be awarded Fail (U), Pass (G) and Pass (VG) with 50% correct answers to the exam will be required for Pass (G) and 75% correct answers will be required for VG. The exam will have components directly from the laboratory reports.

For an approved grade on a full course, both the written exam and the compulsory marks included in the course are required. Regarding the application of the ECTS scale for grades please see Rector's decision 20070528, dnr G 8 1976/07.

9. Course evaluation

Course evaluation is made in relation to the learning objectives and content of the course and is conducted at the end of the course through an individual written questionnaire on the University of Gothenburg's learning platform. Students who participate in or have completed a course should be given the opportunity to anonymously present experiences and views on the course in a course evaluation. A summary of the course evaluation and the teacher's reflection will be made available to the students within a reasonable time after the course. The next time the course is given, the compilation and any actions taken will be presented.

10. Other

Teaching is in English. This is a hybrid course, when possible, to develop skill useful in the modern pandemic and post-pandemic world. Lectures and discussions will take place via Zoom and labs and demos will be in person, as much as possible.

Note: For spring 2022, all those attending lab sessions are expected to wear well-fitting masks over mouth and nose. Any student not following the rules will be asked to leave.